After COVID-19 happened, there was no need to go out, socialize, or attend a
party, so that a lot of humor was added to the circle of friends. "It's finally
time to lie down and contribute to the country." Is the new coronavirus scary?
Terrible, as of February 6 at 24 o'clock died 636 cases; but not terrible, a
total of 1540 cases were discharged, the cure rate is more than double the death
rate.
Specimen Collection Swab
New Coronavirus patients were detected from 321 on January 22, 2020 to 31,268 on February 7, 2020. But from another perspective, is our virus detection capability more accurate? Is our disease treatment more effective?
Five steps to detect new coronavirus
Everyone who goes for a new coronavirus test is full of expectations, hoping that the results will come out earlier, of course, it must also be uneasy. They are anxiously and frightenedly looking forward to the test result. In fact, the test procedure only needs to go through five steps: sampling, sample retention, storage, nucleic acid extraction, and computer testing. This requires rigorous scientific experiments to complete.
Let me first talk about the nucleic acid detection box, which is now used for the detection of new coronavirus. The main detection "magic weapon" in this box is the Specimen Collection Swab. It may be easier to understand how it detects patients.
Use a pharyngeal swab to wipe the posterior pharyngeal wall and bilateral pharyngeal tonsils 5-10 times each, and continuously rotate the swab, this is the first step of the patient's operation;
The second step requires medical staff to retain the sample, immerse the swab head in the cell preservation solution, and immediately tighten the cap after breaking the tail;
The third step is to store, put the sample tube in a sealed bag and send it for inspection in a timely manner. During the inspection process, a strict transportation environment is required and it should be stored at 2-8 degrees Celsius.
Here the doctor's preliminary work is completed, and the next step is to enter the laboratory for testing. Before the test, the virus needs to be inactivated. This effect will destroy the high-level structure of the virus protein and the protein will no longer have physiological activity, so it will lose the ability of infection, disease and reproduction, but the conventional inactivation will not affect the virus protein. The primary structure means that the sequence of the viral protein has not changed.
The fourth step is to perform nucleic acid extraction and extract the nucleic acid from the inactivated virus sample for subsequent detection. The manual method can be used to add physiological saline to the samples one by one. This method needs to increase the detection time by nearly half a minute. You can also use automated equipment, such as nucleic acid extraction instrument.
The fifth step of fluorescent PCR nucleic acid detection, that is, on-machine detection, the extract is subjected to fluorescent PCR amplification reaction, which takes 70-80 minutes. At the end, the whole inspection process is like this.
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