What is the key to nucleic acid detection specimen collection?
Whether collecting nasopharyngeal swabs or oropharyngeal swabs, the depth of collection is the key. The collected nasopharyngeal swabs are not collected deep in the nasal cavity. Most of the cells collected are virus-free cells, which may cause "False negative".
Coronavirus Swab
Let’s take a look at the anatomical differences between the nasopharynx and the oropharynx.
Anatomical structure: The nasal cavity, oral cavity, and throat are correspondingly divided into three parts: the upper, middle and lower parts of the nasopharynx, oropharynx, and laryngopharyngeal, and the nasopharynx is more upper.
1. The sampler held the swab with his right hand (a plastic rod swab with a polypropylene fiber head), and fixed it by pressing the top of the subject's head with his left hand.
2. The swab with the tip slightly bent down enters from the front nostril and goes deep along the bottom of the lower nasal passage slowly and deeply. {Because the nasal passage is curved, the movement should be gentle and slow. Do not use excessive force to avoid traumatic bleeding}.
3. When the tip of the swab reaches the back wall of the nasopharyngeal cavity (with a feeling of touching the wall), leave the swab for a while (about 3 seconds), then gently rotate it one week and slowly take out the swab.
4. You can take another plastic rod swab with polypropylene fiber head to collect the other nostril in the same way, immerse the above 2 swabs into the same tube containing 3ml virus sampling solution, discard the tail, and tighten the tube cap .
5. After being packaged according to biosafety requirements, it should be transported to CDC laboratories at all levels for viral nucleic acid testing as soon as possible.
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